Ted Holman

TitleProfessor
DivisionPhysical & Biological Sciences
DepartmentChemistry & Biochemistry Department
Phone831-459-5884
Email
Web Site Holman Research Web Site
Office250 Physical Sciences Building
Office HoursChem 1A: Tues & Wed 330-430, Chem 146B: By appointment. Group Meeting Tuesday, 12:30 PM to 2 PM
Campus Mail StopChemistry
Mail1156 High Street, 250 PSB
Santa Cruz, CA
95064
picture of Ted Holman

Research Interests

Significance:
Biochemistry is a fascinating and challenging discipline at the interface of biology and chemistry. Our research group utilizes enzymology, site directed mutagenesis, molecular biology and spectroscopy to investigate the biological function of enzymes from a rigorous chemical perspective. Specifically, we study the biological function of lipoxygenase (LOX). Soybean LOX is directly involved in soybean germination and senescence, while human LOX has been implicated in a variety of human diseases, such as asthma, heart disease, stroke and diabetes, to name a few. Therefore, LOX is a critically important enzyme in human health and the Holman laboratory endeavors to understand its enzymatic mechanism, discover novel inhibitors and investigate the role of LOX in human health.

Allosteric Regulation:
There are six LOX isozymes and seven significant fatty acid substrates in the human body. The substrate specificity of each of the LOX isozymes varies from one to another, resulting in a complex array of LOX products being formed in human tissue. However, the Holman laboratory recently discovered that LOX products bind to an allosteric site on LOX, which changes the isozymes substrate specificity. This result is significant because it indicates that the distribution of LOX products can be modified, depending on the enzymatic activity of the six LOX isozymes and the available fatty acid substrates. The Holman laboratory is currently investigating the biochemical properties of the allosteric site and how it may be utilized to increase the production of beneficial LOX products.

Inhibitor Discovery:
Our lab is also interested in the discovery and characterization of novel inhibitors to lipoxygenase. We currently have discovered over 50 unique lipoxygenase inhibitors through screening the marine natural products library of our collaborator, Prof. Phil Crews and the NIH library with our collaborators at the NCGC (Dr. D. Maloney, Dr. A. Simeonov and Dr. A. Jadhav). We have screened these libraries with our high throughput lipoxygenase assay, which has increased our inhibitor discovery dramatically. These inhibitors are then investigated with biochemical and spectroscopic methods to determine how they bind and inhibit lipoxygenase.

Biological Relevance:
The Holman laboratory utilizes the knowledge gained from their biochemical and inhibitory studies to probe LOX in human disease. Currently, the Holman laboratory investigates the role of LOX in Type-1 diabetes (Dr. J. Nadler), blood coagulation (Dr. M. Holinstat), stroke (Dr. K. van Leyen) and fungal-derived inflammation (Dr. S. Kelly). With these collaborations, the Holman laboratory hopes to contribute to the understanding of these important human diseases and possibly identify potent therapeutics.

Biography, Education and Training

B.A., University of California, San Diego
Ph.D., University of Minnesota, Minneapolis
Helen Hay Whitney Fellow, Harvard Medical School

Courses Taught

Chem 1A: General Chemistry
Chem 151AL: Advanced Inorganic Chemistry
Chem 146B: Advanced Inorganic Chemistry laboratory
Chem 256B: Spectroscopy of bioinorganic molecules

Advisees, Post Docs, Graduate Students, Researchers

NameE-Mail AddressPhone Number
Netra Joshi Joshi
John B Jameson
Michelle Marie Armstrong
Auric Anthony Sowell-Kantz